Abstract :
AIM OF THE STUDY:
Gimjeng and Chakapat lychee (Litchi chinensis Sonn.) were evaluated for
hepatoprotective activity on CCl(4)-induced hepatotoxicity in rats. MATERIALS
AND METHODS: Fruit pulp extracts of the lychees were examined for vitamin C,
phenolic contents, anti-lipid peroxidation activity and hepatoprotective
effect. Male Wistar albino rats were intraperitoneally injected (ip) with
CCl(4) (2 ml/kg), then were orally administered (po) with silymarin (100mg/kg),
and Gimjeng or Chakapat extracts (100 and 500 mg/kg). After ten days, the rats
were sacrificed and their livers were examined histopathologically and
immunohistochemically. Their serum glutamate-pyruvate transaminase,
glutamate-oxalate transaminase, and alkaline phosphatase activities were
analyzed. Apoptotic activity of the livers was assessed quantitatively.
RESULTS: The Gimjeng and Chakapat extracts showed the contents of vitamin C
(1.2+/-0.6 and 4.3+/-0.1mg/100g) and phenolics like trans-cinnamic acid and
pelargonidin-3-O-glucoside (9.80+/-0.21 and 19.56+/-0.4 mg GAE/g extract,
respectively), and trolox equivalent antioxidant capacity (TEAC) values (11.64
and 9.09 g/mg trolox), respectively. The Gimjeng as compared to the Chakapat
demonstrated a better antioxidant activity as revealed by anti-lipid
peroxidation activity with the TEAC values. Administration of CCl(4) in rats
elevated the serum GPT, GOT, and ALP level whereas silymarin, Gimjeng and
Chakapat extracts prevented these increases significantly. Significant decrease
of apoptotic cells together with restoration of morphological changes confirmed
the hepatoprotective effect in the CCl(4)-induced rats pretreated with the
extracts. CONCLUSION: Antioxidant properties of the Gimjeng and Chakapat
lychees as evidenced by the vitamin C and phenolic compounds, anti-lipid
peroxidation and anti-apoptosis could explain the hepatoprotective effects in
CCl(4)-induced hepatotoxicity.
Materials and methods
Fruit
pulp extracts of the lychees were examined for vitamin C, phenolic
contents, anti-lipid peroxidation activity and hepatoprotective effect.
Male Wistar albino rats were intraperitoneally injected (ip) with CCl4 (2 ml/kg), then were orally administered (po)
with silymarin (100 mg/kg), and Gimjeng or Chakapat extracts (100 and
500 mg/kg). After ten days, the rats were sacrificed and their livers
were examined histopathologically and immunohistochemically. Their serum
glutamate–pyruvate transaminase, glutamate–oxalate transaminase, and
alkaline phosphatase activities were analyzed. Apoptotic activity of the
livers was assessed quantitatively.
Results
The Gimjeng and Chakapat extracts showed the contents of vitamin C (1.2 ± 0.6 and 4.3 ± 0.1 mg/100 g) and phenolics like trans-cinnamic acid and pelargonidin-3-O-glucoside
(9.80 ± 0.21 and 19.56 ± 0.4 mg GAE/g extract, respectively), and
trolox equivalent antioxidant capacity (TEAC) values (11.64 and
9.09 g/mg trolox), respectively. The Gimjeng as compared to the Chakapat
demonstrated a better antioxidant activity as revealed by anti-lipid
peroxidation activity with the TEAC values. Administration of CCl4
in rats elevated the serum GPT, GOT, and ALP level whereas silymarin,
Gimjeng and Chakapat extracts prevented these increases significantly.
Significant decrease of apoptotic cells together with restoration of
morphological changes confirmed the hepatoprotective effect in the CCl4-induced rats pretreated with the extracts.
Conclusion
Antioxidant
properties of the Gimjeng and Chakapat lychees as evidenced by the
vitamin C and phenolic compounds, anti-lipid peroxidation and
anti-apoptosis could explain the hepatoprotective effects in CCl4-induced hepatotoxicity.
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