Abelmosichi
Corolla
(Huangshukuihua)
Sunset Abelmoschus
Flower
Sunset Abelmoschus
Flower is the dried corolla of Abelmoschus manihot (L ) Medic. (Fam
Malvaceae). The drug is collected at flowering in summer and autumn, and dried
in time.
Description:
Mostly crum'pled
arid broken, when whole, petal triangular broad-obovate, 7-19 cm long, 7-12 cm
wide, externally with longitudinal winkles, radial, pale green, margin slightly
sinuous; the inner surface base purpish-brown, stameus numerous, aggregated to
a tube, 1.5-2. 5 cm long, anther almost sessible, stigma purplish- black,
whorl-orbiculate 5-lobed. Odour, slightly aromatic; taste, sweet and weak.
Identification:
(1) Powders: Pale
yellow to brownish- yellow, epidermal cells of corolla subrectangular or
irregular, anticlinal walls slightly sinuous. Pollen grains subrounded, about
170 µm in diameter, scattered with 3240 germinal pores, externally with
spines, whole glandular hairs long-conical, 510-770 pm long, head of glandular
hair somewhat long clavate, 6-14 cellular, glandular stalk 3 cellular,
containing purplish-red secretion; non-glandular hairs unicellular, 140-180 pm
long, walls smooth. Cells of inner walls of pollen sac subrectangular in
facture surface, walls stripe-like thickened, subpolygonal in surface view,
anticlinal walls bead-like thickened. Clusters of calcium oxalate minute, 9-19
pm in diameter, angular acute.
(2) To 1 g of the
powder add 20 ml of 0.18% solution of hydrochloric acid in ethanol, heat under
reflux for 1 hour, filter, concentrate the filtrate to 5 ml and use as the test
solution. Dissolve quercetin CRS in ethanol to produce a solution containing 0.5
mg per ml as the reference solution. Carry out the method for thin layer
chromatography (Appendix VI B), using silica gel G mixed with 0.5% solution of
sodium hydroxide as the coating substance and a mixture of toluene, ethyl acetate
arid formic acid (5 : 4 : 1) as the mobile phase. Apply separately 1 pi of each
of the above two solutions to the plate. After developing and removal of the
plate, dry it in air, spray with aluminum chloride TS, and examine under
ultraviolet light at 365 nm. The fluorescent spot in the chromatogram obtained
with the test solution corresponds in position and colour to the spot in the
chromatogram obtained with the reference solution.
Water:
Not more than 12.0
per cent (Appendix IX H, method 1).
Total
ash:
Not more than 8.0
per cent (Appendix IX K). Acid-insoluble ash Not more than 2.0 per cent
(Appendix IX K).
Extractives Carry
out the method for determination of ethanol-soluble extractives (Appendix X A,
the cold maceration extraction method), not less than 18.0 per cent, using
ethanol as the solvent.
Assay:
Carry out the method for high performance
liquid chromatography (Appendix VI D).
Chromatographic
system and system suitability Use
octadecylsilane bonded silica gel as the stationary phase and a mixture of
acetonitrile and 0.1% phosphoric acid solution (15:85) as the mobile phase. As
detector a spectrophotometer set at 360 nm. The number of theoretical plates of
the column is not less than 10 000, calculated with reference to the peak of
hyperoside.
Reference
solution Dissolve
a quantity of garlicin CRS in methanol, accurately weighed, to produce a
solution containing 0.1 mg per ml as the reference solution.
Test solution Weigh accurately 0.2 g of the
powder (through No. 4 sieve) in a 25 ml measuring flask, add 15 ml of methanol,
ultrasonicate (power, 250 W; frequency, 30 kHz) for 30 minutes, cool and
replenish the loss of weight with methanol, mix well, filter and use the
successive filtrate as the test solution.
Procedure Inject accurately 10 µl of each
of the reference solution and the test solution, into the column, and calculate
the content.
It contains not
less than 0.50 per cent of hyperoside (C21H20O12)
, calculated with reference to the dried drug.
Prepared
slices
Processing:
Eliminate foreign
matter as well as dust and scrap.
Description
and Identification:
As required for the
crude drug.
Water,
Total Ash, Acid-insoluble ash, and Extractives:
As required for the
crude drug.
Assay:
As required for the
crude drug.
Property
and Flavor:
Cold; sweet.
Meridian
tropism:
Kidney and bladder
meridians.
Actions:
To clear and drain
dampness-heat, disperse swelling and remove toxin.
Indications:
Obstruction of
dampness-heat, turbid stranguria, edema; Topical application, abscesses and
cellulitis, swelling and toxin, scald and bum.
Administration
and dosage:
10-30 g; Ground
into powder for oral administration, 3-5 g; Appropriate amount for topical
application, ground into powder for applyment.
Contraindication:
Used cautiously for
pregnant woman. Storage Preserve in a dry place.
