Navid Adnani, Cole R. Michel, and Tim S. Bugni*
Pharmaceutical Sciences Division, University of Wisconsin−Madison, 777 Highland Avenue, Madison, Wisconsin 53705, United States
J. Nat. Prod., Article ASAP
DOI: 10.1021/np300034c
Publication Date (Web): April 3, 2012
Copyright © 2012 The American Chemical Society and American Society of Pharmacognosy
A lack of good methods for absolute quantification of natural products
has limited the accuracy of high-throughput screening. Many currently
used methods for quantification are either too slow or not amenable to
the structural diversity of natural products. Recent developments in
low-temperature evaporative light scattering detectors (ELSD-LT) have
overcome several historical limitations of ELSDs, including analyte
decomposition and low sensitivity. Primarily, ELSDs have been used for
relative quantification and detection of compounds that lack a UV
chromophore. In this study, we employ an ELSD-LT for absolute
quantification of natural products. Calibration curves were constructed
using a weighted least-squares analysis for a diverse set of natural
products and other compounds. An average calibration curve was evaluated
for the “universal” quantification of natural products. Optimization of
ELSD-LT hardware and parameters improved sensitivity and throughput and
established the utility of ELSD-LT for quantification of large natural
product libraries.
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